DETECTION AND SPECIES IDENTIFICATION OF PLASMODIUM USING NESTED PCR AND DIAGNOSIS OF P.FALCIPARUM, P.VIVAX, P.KNOWLESI AND MIXED INFECTION IN SOUTH NIAS REGENCY, NORTH SUMATERA, INDONESIA

Research Journal of Pharmaceutical, Biological and Chemical Sciences

Harurikson Lumban Tobing, Rahmiana Zein, Endang Purwati, Free Agustina P. Sinaga, Edy Fachrial, S.Si., M.Si., Sumaryati Syukur

Senin, 11 September 2017 11:22 | Sudah dibaca 1080 kali

The aim of this study was to determine the sensitivity and specificity of diagnostic test to infection of P.falciparum, P.vivax, P.malariae, P.ovale and P.knowlesi in endemic areas in South Nias regency using Nested2 PCR method. The number of subject in this study were 98 people, consist of 50 female subjects (51%) and 48 male subjects (49%). Majority of the samples were 15 years old to 59 years old (41,8%) and 5 years old to 14 years old (36,7%) and the least were over 60 years old (6,1%). Most symptoms were fever (74,5%), headache (72,4%) and splenomegaly (30,6%). The result of the study with Nested-2 PCR on parasite P.falciparum and P.vivax showed sensitivity 100%, specificity 100%, NPP 95.83%, NPN 100%, positive likelihood ratio 5,17%, negative likelihood ratio 0,31% and the prevalence of P.knowlesi 0,97%. It concluded that Nested-2 PCR method has a high sensitivity and specificity so it can be used as diagnostic support for malaria cases.

Kata Kunci: Malaria, Nested 2 PCR, Plasmodium knowlesi, sensitivity, specificity

PENULIS