Comparison Activities of Peel and Extract of Lime (Citrus amblycarpa) as Antioxidant and Antielastase

American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS)

Sabtu, 14 Maret 2020 11:14 | Sudah dibaca 576 kali

Aging of the skin is a physiological process that occurs in all living things, along with increasing age, hormones, nutrition, and exposure to sunlight both directly and indirectly. This process will form Reactive Oxygen Species ROS, free radicals that interfere with one of the most important enzymes in the cell matrix, namely the elastase enzyme. Free radicals can be neutralized by the presence of antioxidants. Lime plants have natural phytochemical compounds that act as antioxidants and anti-aging. This study was to compare the antioxidant activity and anti-elastase from the peel and lime seed extract (Citrus amblycarpa) by conducting H2O2 trapping tests and inhibition of elastase. The test results of antioxidant activity and anti-aging trapping of H2O2 lime seed extract had an IC50 value lower than lime peel extract, with yields of 194.82 μg / ml better than those of lime skin extract of 246.84 μg / ml. The results of the elastase inhibition activity test showed that lime peel extract had a lower IC50 value of 36.94 μg / ml compared to lime seed extract with an IC50 value of 62.39 μg / ml. A lime seed extract has antioxidant activity through better H2O2 trapping compared to lime peel extract while lime skin extract has antiaging activity through inhibition of elastase which is better than lime seed extract.

Kata Kunci: Lime extract; Antioxidant ; Antielastase