Antioxidant and Anti-Inflammatory activity of Chlorogenic Acid on LeadInduced Fibroblast Cells

The 1st International Seminar on Smart Molecule of Natural Resources

Rabu, 18 Maret 2020 09:20 | Sudah dibaca 521 kali

Lead (Pb) can be a cause of oxidative stress, because it is a toxin found in the environment and can have a harmful effect on the body. increased reactive oxygen species (ROS) and nitrogen species (RONS) levels can cause cellular aging and inflammation. natural compound found in plant is one of the sources of antioxidant and anti-inflammatory agent that has ability to prevent aging including chlorogenic acid (CA). Cytotoxicity assay of CA against fibroblast cells (BJ) was conducted using MTS (3-4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The intracellular ROS levels were detected by flow cytometry using a DCF-DA fluorescent probe. BJ cells were incubated at 370C, 5% CO2, treated by 25 and 6.25 µg/ml CA for 4 h and followed by 400 µg Pb for 3 days. The anti-inflammatory potential was determined using ELISA to measure IL10 and TNFα. CA at 3.13-25 µg/ml were nontoxic to the BJ cells. CA treatment at 6.25 and 25 µg/ml was capable to reduce the accumulation of ROS in lead-induced BJ cells. CA at 6.25 and 25 µg/ml increased IL10 and reduced TNFα compared to positive control (lead-induced cells). This study shows that chlorogenic acid has the potential as protective effect through suppression of ROS levels and have anti-inflammatory properties related to Pb poisoning.

Kata Kunci: lead, reactive oxygen species, chlorogenic acid, IL10, TNFα