Antioxidant and Tyrosinase Enzyme Inhibition Activity of Lime Peel and Seed Ethanol Extract

American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS)

Rabu, 18 Maret 2020 10:19 | Sudah dibaca 570 kali

UV Light exposure and Oxidative stress can cause the accumulation of free radicals and increase melanogenesis. The various herbal plant enriches Indonesia. One of them is lime that content various phytochemicals. This study was aimed to explore antioxidants property and tyrosinase enzyme inhibition activity of lime peel and lime seed. This study used ethanol extract from lime peel and seed. The antioxidant activity was evaluated by the ABTS reduction assay, while tyrosinase enzyme inhibitor activity by tyrosinase enzyme from fungi. The results of this study showed that the IC50 Value of Lime Seed (14.09 ± 0.88 μg/mL) was lower than lime peel (20.16 ± 0.23 μg/mL). Meanwhile, the IC50 value against tyrosine enzyme of Lime Peel (34.23 ± 0.93 μg/mL) was lower than lime seed (67.88 ± 3.73μg/mL). Hence lime peels potentially become a tyrosinase enzyme inhibitor agent while the lime seed has better antioxidant potential than the peel.

Kata Kunci: lime; peel; seed; ABTS; tyrosinase enzyme.